Part:BBa_K533005:Design

OmpA-GFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1419
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

T7 promoter driven OmpA-GFP fusion protein. His tag is inserted between OmpA and GFP for easier detection in Western Blotting.

Source

OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1